Mt genomes also provide a rich source of markers to aid molecular epidemiological and ecological studies of key parasites. Mitochondrial (mt) genomics has significant implications in a range of fundamental areas of parasitology, including evolution, systematics, and population genetics as well as explorations of mt biochemistry, physiology, and function. Jex, Aaron R Littlewood, D Timothy Gasser, Robin B Sequencing and annotation of mitochondrial genomes from individual parasitic helminths.
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Comparisons between crack growth predicted by using the LOSEQ-programme and experimentally observed data are presented. It considers not only the maximum peak of a load sequence to effect crack growth but also all other loads of the history according to a generalised memory criterion. International Nuclear Information System (INIS)Ī new analytical model for predicting non-linear crack growth is presented which takes into account the retardation as well as the acceleration effects due to irregular loading. Model for predicting non-linear crack growth considering load sequence effects (LOSEQ) This approach also identified new sequences corresponding to as-yet unknown organisms similar to tropical Theileria species.Our findings demonstrate the capability of NGS to produce an inventory of live tick-borne parasites, which could potentially be transmitted by the ticks, and uncovers unexpected parasites in Western Europe. This is the first time that these last two species have been identified in France. Four Babesia species were identified, three of which were zoonotic: B.
Sequences corresponding to parasites from two distinct genera were recovered in Ixodes ricinus ticks collected in Eastern France: Babesia spp. Assessment of pathogens carried by wild ticks must be performed without a priori, to allow for the detection of new or unexpected agents.We evaluated the potential of Next-Generation Sequencing techniques (NGS to produce an inventory of parasites carried by questing ticks. Identification of parasitic communities within European ticks using next-generation sequencing.įull Text Available Risk assessment of tick-borne and zoonotic disease emergence necessitates sound knowledge of the particular microorganisms circulating within the communities of these major vectors. We report the draft genome sequence of the root-knot nematode Meloidogyne incognita, a biotrophic parasite of many crops, including tomato, cotton and coffee. Plant- parasitic nematodes are major agricultural pests worldwide and novel approaches to control them are sorely needed. Genome sequence of the metazoan plant- parasitic nematode Meloidogyne incognitaĪbad, P. glycines genes with putative functions in parasitism, we have reduced the Furthermore, in an effort to identify additional H. Conclusion We have established an expression atlas for all known H. This is the first study combining sequence analyses of a substantial EST dataset with microarray expression data of all major life stages (except adult males for the identification and characterization of putative parasitism-associated proteins in any parasitic nematode. Analyses of the expression profiles of these genes allowed the formulation of hypotheses about potential roles in parasitism. glycines genes, some of which also contained a signal peptide. glycines parasitism proteins have strongest similarity to proteins of plants and microbes, we searched for predicted protein sequences that showed their highest similarities to plant or microbial proteins and identified 156 H. Furthermore, because some of the known H.
glycines parasitism-associated genes, we isolated 633 genes encoding secretory proteins using algorithms to predict secretory signal peptides. Targeting the identification of additional H.
These insights were gained by analyzing our gene expression dataset from experiments using the Affymetrix Soybean Genome Array GeneChip, which contains probeset sequences for 6,860 genes derived from preparasitic and parasitic H.
Results We present here the expression patterns of all previously described parasitism genes of the soybean cyst nematode, Heterodera glycines, in all major life stages except the adult male. These proteins are the translation products of parasitism genes and are secreted molecular tools that allow cyst nematodes to infect plants. Sequence mining and transcript profiling to explore cyst nematode parasitismĭirectory of Open Access Journals (Sweden)įull Text Available Abstract Background Cyst nematodes are devastating plant parasites that become sedentary within plant roots and induce the transformation of normal plant cells into elaborate feeding cells with the help of secreted effectors, the parasitism proteins.